Description
Description: This assay employs a two-site sandwich ELISA to quantitate FIGN in samples. An antibody specific for FIGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FIGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FIGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FIGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The mouse mutation 'fidget' arose spontaneously in a heterogeneous albino stock. This mutant mouse is characterized by a side-to-side head-shaking and circling behavior, due to reduced or absent semicircular canals. Fidget mice have small eyes, associated with cell cycle delay and insufficient growth of the retinal neural epithelium. They also have lower-penetrance skeletal abnormalities, including pelvic girdle dysgenesis, skull bone fusions, and polydactyly. The fidget mutation had been mapped to mouse chromosome 2. AAA proteins are molecular chaperones that facilitate a variety of functions, including membrane fusion, proteolysis, peroxisome biogenesis, endosome sorting, and meiotic spindle formation. the fidget mutation in the mouse was due to a retrotransposon insertion in intron 2, interfering with normal RNA processing